The Korus™ counterflow centrifugation system operates on the principle of elutriation. Elutriation is a process for separating cells based on their size, shape and density using a stream of fluid flowing in the opposite direction to the centrifugal force.
The benefits of elutriation are:
Up to 88.6% monocyte recovery and 75% monocyte purity. Isolation of monocytes
for downstream processes such as dendritic cell (DC) maturation.
1 LOADING
Cells from the input bag enter the chamber and are held there as excess fluid along with most of the platelets and a minor portion of RBCs flow to waste.
2 ELUTRIATION
Media flows into the chamber which causes the lymphocytes and many of the RBCs to leave the chamber, these are sent to either the transfer fraction bag or waste.
3 HARVEST
With most of the other cell types removed, the remaining monocytes are flushed from the chamber to the harvest bag.
*Standard media exchange capability: Korus™ streamlines the manufacturing workflow with the media exchange as well as volume reduction as a standard feature on Korus. Korus can help you achieve the desired cell concentration, volume, and suspension media through a fully automated process.
Up to 85.2% lymphocyte recovery and 90.3% purity, providing a cleaner starting material for CAR-T or TIL manufacturing. Can also be used for PBMC enrichment.
Cells from the input bag enter the chamber and are held there as excess fluid along with most of the platelets and a minor portion of RBCs flow to waste.
Media flows into the chamber which causes the lymphocytes and many of the RBCs to leave the chamber, these are collected in the ‘fraction bag’.
The concentrated lymphocytes are then flushed from the chamber into the harvest bag using a small volume of media.
*Standard media exchange capability: Korus™ streamlines the manufacturing workflow with the media exchange as well as volume reduction as a standard feature on Korus. Korus can help you achieve the desired cell concentration, volume, and suspension media through a fully automated process.
Maximizing cell recovery and contaminant removal
*Standard media exchange capability: Korus™ streamlines the manufacturing workflow with the media exchange as well as volume reduction as a standard feature on Korus. Korus can help you achieve the desired cell concentration, volume, and suspension media through a fully automated process.
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