Counterflow Centrifugation Systems

Korus™

Elutriation. Washing and concentration. Media exchange. One System.

What is elutriation?

The Korus™ counterflow centrifugation system operates on the principle of elutriation. Elutriation is a process for separating cells based on their size, shape and density using a stream of fluid flowing in the opposite direction to the centrifugal force.

The benefits of elutriation are:

Separation of target from non-target cells
Gentle production of a non-pelleting cell bed
Separation of viable and non-viable cells

Korus supports multiple cell processing protocols across diverse cell types.

Monocyte elutriation

Enrich monocytes with up to 94% recovery and 80% purity, with 99.7% platelet depletion and 90% red blood cell removal in a single 35 minute run.

Lymphocyte elutriation

Enrich lymphocytes with up to 91% recovery and 90% purity delivering high-quality starting material for CAR-T and PBMC based workflows.

Cell wash and concentrate

Replace media and remove contaminants in a single automated step, maximizing cell recovery while delivering your target concentration and volume in a user-selected buffer.

Monocyte elutriation

Enrich monocytes with up to 94% recovery and 80% purity delivering a high-quality starting population for dendritic cell (DC) differentiation and other monocyte-dependent workflows.

• Lymphocytes • Monocytes • RBCs • Platelets

1 LOADING

Cells from the input bag enter the chamber and are held there as excess fluid along with most of the platelets and a minor portion of RBCs flow to waste.

2 ELUTRIATION

Media flows into the chamber which causes the lymphocytes and many of the RBCs to leave the chamber, these are sent to either the transfer fraction bag or waste.

3 HARVEST

With most of the other cell types removed, the remaining monocytes are flushed from the chamber to the harvest bag.

*Standard media exchange capability: Korus™ streamlines the manufacturing workflow with the media exchange as well as volume reduction as a standard feature on Korus. Korus can help you achieve the desired cell concentration, volume, and suspension media through a fully automated process.

Lymphocyte elutriation

Up to 91% lymphocyte recovery and 90% purity providing a high-quality starting material for CAR-T manufacturing and PBMC enrichment.

• Lymphocytes • Monocytes • RBCs • Platelets

1 LOADING

Cells from the input bag enter the chamber and are held there as excess fluid along with most of the platelets and a minor portion of RBCs flow to waste.

2 ELUTRIATION

Media flows into the chamber which causes the lymphocytes and many of the RBCs to leave the chamber, these are collected in the ‘fraction bag’.

3 CONCENTRATION

Lymphocytes from the fraction bag are loaded into the chamber where they are held and concentrated down much like a filter. During this step the remaining platelets and up to 50% of the RBCs are removed.

4 HARVEST

The concentrated lymphocytes are then flushed from the chamber into the harvest bag using a small volume of media.

Cell wash and concentrate

Maximizing cell recovery and contaminant removal

• Cells

1 LOADING

Cells from the input bag are loaded and concentrated in the chamber. Excess fluid from the input bag flows to waste.

2 WASH

With the cells still suspended in the chamber, the remaining starting fluid is completely replaced with a second media. All primary media and any contaminants flow to waste.

3 HARVEST

Once the media in the chamber is replaced the concentrated cells flow from the chamber to the harvest bag and are suspended in a user selectable volume of secondary media.

See if Korus fits your protocol.

Send us your starting material, target output, and downstream needs. We’ll show you which Korus configuration fits.