KorusTM
Elutriation. Washing. One system.
Prepare cleaner cell populations
with the Korus™ system
Monocyte elutriation
Up to 78.6% monocyte purity and 70.1% recovery. Isolation of monocytes for downstream processes such as dendritic cell (DC) maturation.
Lymphocytes
Monocytes
1
INPUT
Lymphocytes and monocytes are loaded into the chamber from the input bag.
2
ELUTRIATION
Lymphocytes are eluted from the top of the chamber into the waste bag with the monocytes remaining at the bottom of the chamber.
3
HARVEST
Monocytes flow from bottom of chamber to harvest bag.
Lymphocyte elutriation
Up to 85.2% lymphocyte recovery and 90.3% purity, providing a cleaner starting material for CAR-T or TIL manufacturing.
Lymphocytes
Monocytes
1
INPUT
Lymphocytes and monocytes enter the chamber from the input bag. Excess input solution flows to waste.
2
ELUTRIATION
Lymphocytes leave the top of the chamber to the fraction bag with monocytes remaining at the bottom of the chamber.
3
CONCENTRATION
Lymphocytes are loaded into the chamber from the fraction bag. Excess buffer flows to waste.
4
HARVEST
Lymphocytes flow from bottom of chamber to harvest bag.
Cell wash
Maximizing cell recovery and contaminant removal.
Cells
1
INPUT
Cells are loaded into the chamber from the input bag. Excess input solution flows to waste.
2
WASH
Buffer flows through the chamber exchanging medium that the cells are suspended in and removing contaminants.
3
HARVEST
Cells flow from bottom of chamber to harvest bag.
Use of Korus™ in manufacturing processes
The steps in the process highlighted in green are where the Korus™ would be used.
CAR-T manufacturing process
DC manufacturing process
TIL manufacturing process
IPSC-derived therapy manufacturing process
What is elutriation?
The Korus™ counterflow centrifugation system operates on the principle of elutriation. Elutriation is a process for separating cells based on their size, shape and density using a stream of fluid flowing in the opposite direction to the centrifugal force.
The benefits of elutriation are:
- Separation of target from non-target cells
- Gentle production of a non-pelleting cell bed
Stages in the lymphocyte elutriation process:
1
Loading
Transfer of input cells into chamber(s).
3
Transfer fraction
Transfer of lymphocytes from fraction bag to chamber for concentration.
2
Elutriation
Increased counterflow removes smaller cells (lymphocytes) from chamber(s) to fraction bag.
4
Harvest
Transfer of cells from chamber to harvest bag in a user-defined volume.
Lymphocytes
Cells
The Korus system is for research, laboratory or further manufacturing use only. It is not intended as a medical device in therapeutic or diagnostic procedures. Customers are responsible for validating the use of Korus within their process or therapy. Consult all instructions, terms and warnings prior to use of the solutions and products. Failure to do so can result in damage or serious injury, including death. Ready-to-run turnkey systems may require minor configuration before sale and use depending on customer needs.