Elutriation. Washing. One system.
Up to 78.6% monocyte purity and 70.1% recovery. Isolation of monocytes for downstream processes such as dendritic cell (DC) maturation.
Lymphocytes and monocytes are loaded into the chamber from the input bag.
Lymphocytes are eluted from the top of the chamber into the waste bag with the monocytes remaining at the bottom of the chamber.
Monocytes flow from bottom of chamber to harvest bag.
Up to 85.2% lymphocyte recovery and 90.3% purity, providing a cleaner starting material for CAR-T or TIL manufacturing.
Lymphocytes and monocytes enter the chamber from the input bag. Excess input solution flows to waste.
Lymphocytes leave the top of the chamber to the fraction bag with monocytes remaining at the bottom of the chamber.
Lymphocytes are loaded into the chamber from the fraction bag. Excess buffer flows to waste.
Lymphocytes flow from bottom of chamber to harvest bag.
Maximizing cell recovery and contaminant removal.
Cells are loaded into the chamber from the input bag. Excess input solution flows to waste.
Buffer flows through the chamber exchanging medium that the cells are suspended in and removing contaminants.
Cells flow from bottom of chamber to harvest bag.
Use of Korus™ in manufacturing processes
The steps in the process highlighted in green are where the Korus™ would be used.
CAR-T manufacturing process
DC manufacturing process
TIL manufacturing process
IPSC-derived therapy manufacturing process
What is elutriation?
The Korus™ counterflow centrifugation system operates on the principle of elutriation. Elutriation is a process for separating cells based on their size, shape and density using a stream of fluid flowing in the opposite direction to the centrifugal force.
The benefits of elutriation are:
- Separation of target from non-target cells
- Gentle production of a non-pelleting cell bed
Stages in the lymphocyte elutriation process:
Transfer of input cells into chamber(s).
Transfer of lymphocytes from fraction bag to chamber for concentration.
Increased counterflow removes smaller cells (lymphocytes) from chamber(s) to fraction bag.
Transfer of cells from chamber to harvest bag in a user-defined volume.